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GLA-specific single-guide RNA (sgRNA) was prepared to introduce deletion mutations in exon 1 of the GLA gene, as shown in Supplementary Fig

Tony reveals that one key area they're exploring is mood effects, while Katie adds they're also strengthening claims around motor function and developing sports-specific studies

The following reaction system was used: 1 ng pGEX4T-2 plasmid, 50 mM Tris-HCl (pH 7.8), 5 mM MgCl 2 , 10 mM 2-mercaptoethanol, 10 g/ml BSA, 1 ng forward primer FP1 and 1 ng reverse primer RP1, 20 M dNTP, 1 M dideoxynucleotides (ddNTP), 2 units DNA Polymerase I (Invitrogen), and ddH 2 O to the reaction volume of 100 l

Rao and Zheng, 2025

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