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Confirmation of this model would require use of additional phospho-specific antibodies, such as those used recently to demonstrate that pS232 primes phosphorylation at S235 and S238 27,28 , an observation consistent with our findings

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Final selection based on best set of parameters is presented in the (Supplementary Materials 2 Table S1)

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ACTB, B2M, and GAPDH exhibited the lowest geNorm M values and were thus selected for normalization of Cq values