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Quantitative real-time polymerase chain reaction (qRT-PCR) was performed on a Bio-Rad CFX96 instrument using SYBR Green-based qPCR (Bio-Rad, 1725275)

Pharmacokinetics: given i.v

IGF-1 causes muscle cell hyperplasia, which is an actual splitting and forming of new muscle cells

Ipamorelin is supplied as a white to off-white lyophilised solid for reconstitution at the bench with a research solvent such as bacteriostatic water

Whereas CJC-1295 with DAC achieves half-lives of several days via albumin binding, ipamorelin is a short-acting peptide with a tightly limited time window

These can be exact replicas (bioidentical) or altered to enhance stability, potency, or half-life