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The purified PCR product of linearized pFliTrx (without the fusion junction) was used to link it to the DNA library with T4 ligase

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Additionally, untargeted metabolomics analysis showed that the differentially expressed metabolites were primarily correlated with lipid metabolism, including steroid hormone biosynthesis (cholesterol sulfate), primary bile acid biosynthesis (cerebrosterol), and sphingolipid metabolism (phytosphingosine) between the control and TYGM groups

It is worth noting that ginger capsules are dietary supplements and are not regulated by the FDA

For instance, a 12-ounce beer is equivalent to one standard drink