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Clin Infect Dis 17:S309S315 Palella FJ, Baker RK, Moorman AC et al (2006) Mortality in the highly active antiretroviral therapy era: changing causes of death and disease in the HIV outpatient study

After the mixture was washed 5 times with a buffer composed of 50 mM HEPES-KOH (pH 7.5), 100 mM NaCl, 1% Triton X-100, 0.5% deoxycholate, 0.1% SDS, 1 mM EDTA, and a protease inhibitor cocktail (EDTA-free), bound proteins were eluted with 0.1 M citrate and subjected to Western blotting with an anti-Kras antibody (1:250 dilution