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Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark

Zou Z, Hu W, Kang F, Xu Z, Li Y, Zhang J, et al

Plasmid construction The plasmid pBHRSF184 (Addgene plasmid #89482), which expresses the N-terminally His-tagged SUMO-OaAEP1 [33] fusion protein, was used as a template for the generation of SUMO-MT3 and SUMO-LPETG-MT3 plasmids