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In short, sterol fractions from 40,000 young adults were resuspended in chloroform/methanol (2:1 v/v) and injected into a VARIAN CP-3800 Gas Chromatograph equipped with a Factor Four Capillary Column VF-5 ms (30 m 0.25 mm ID DF = 0.25) and analyzed by a Varian 320 MS triple quadrupole with electron energy set to 70 eV at 200 C and the transfer line at 280 C

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