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A low result is obviously a big red flag, but it's crucial to know that this test alone doesn't always tell the whole story

Binding dCas9 to the transcription start site (TSS) of a gene can block the start of transcription, thereby inhibiting gene expression (Figure 1C)

In presence of 3 or 4 mM MG, none of the cells were able to grow

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This limitation is particularly relevant in rice, where recombination rates vary markedly along chromosomes, such that any single kb cM 1 conversion represents an approximation rather than a precise local measure [ 88

[DOI] [PMC free article] [PubMed] [Google Scholar] 280.Lee K., Conboy M., Park H.M., Jiang F., Kim H.J., Dewitt M.A., Mackley V.A., Chang K., Rao A., Skinner C