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In rodent models, dietary restrictions in SAA also showed beneficial effects, including improvements in insulin secretion, modulation of the SAA signaling pathway to mitigate insulin resistance, regulation of glucose homeostasis, alterations in lipid metabolism, reductions in oxidative stress and inflammation, as well as changes in hormone-related factors [42]

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Briefly, the indicated cells were plated in 12-well plates and treated with different concentrations of cytotoxic compounds for the indicated times

This mixture was filled in the affinity column and washing of the column was done by 20 mM Tris-Cl and 20 mM imidazole (pH 7.4)

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However, by inhibiting the genes encoding metabolic enzymes (including HK2, FASN, and GLS1) as well as thyroid differentiation markers (such as NIS and TSHR), DNA methylation systematically reprograms the metabolic network of TC [10, 11]