glutathione redox state in cerebrospinal fluid Dysregulation of Homeostasis Neurodegenerative Diseases Glutathione redox cycle. In the

Figura 1: ciclo vitale dello Schistosoma Figura 2: una coppia di Schistosomi (la femmina e' ospitata nel canale ginecoforo del maschio) vista col microscopio elettronico a scansione Figura 3: la larva cercaria We studied the following enzymes/proteins form Schistosoma mansoni (and haematobium) that have potential interest as vaccine antigens or drug targets: - Glutathione S-transferase (GST) - Cyclophilin (Cyp) - Fatty Acid Binding Protein (FABP) - Thioredoxin Glutathione Reductase (TGR) - Thioredoxin (Trx) - Glutathione peroxidase (Gpx) - Thioredoxin peroxidase (peroxiredoxin, Prx) Thiol reductases in Schistosoma parasites - In all organisms thiols play a crucial metabolic role in the detoxification of oxidizing substances (thioredoxin peroxidase, glutathione peroxidase, glutathione S-transferase) and in the bisynthesis of DNA (ribonucleotide reductase)

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After 4 days, the TS2/16 scFv-Fc was captured from the conditioned media with CaptivA HF protein-A affinity resin (Repligen, CA-PRI-0100) and eluted with IgG elution buffer (Thermo Scientific) containing 300 mM NaCl and neutralized with 100 mM Tris pH 8.0 to reach eventually pH 7.0