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Subsequently, the samples were sonicated in an ice-bath for 30 min, then 200 L of water was added and mixed, followed by incubation at room temperature for 10 min

Additionally, empagliflozin (EMPA), a sodium-glucose co-transporter 2 (SGLT2) inhibitor, has been shown to inhibit IPF by enhancing autophagy and modulating the NRF2/HO-1 signaling pathway [121]

The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)

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& Dang, C

All-transretinoic acid ameliorates bleomycin-induced lung fibrosis by downregulating the TGF-1/Smad3 signaling pathway in rats