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[DOI] [Google Scholar] Findei S

To test if even higher intracellular glutathione levels could be achieved and if this might lead to increased tolerance, we overexpressed the genes from two bacterial genera and assessed the recombinants in simultaneous saccharification and fermentation (SSF) with steam pretreated spruce hydrolysate containing 10% solids

Ice-crystal formation during each freeze-thaw cycle inflicts mechanical damage on peptide chains, and pre-splitting eliminates the cumulative loss that comes from thawing one vial multiple times

Reducing conditions (trace metals, UV light, repeated oxygen exposure from freeze-thaw cycling) can disrupt these disulfide bonds, unfolding the peptide

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