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The criteria for exclusion were as follows: rats with signs of external ear disorders (impacted earwax, tumors, external acoustic meatus edema, and hyperemia), rats with middle ear disorders (opacification, hyperemia, and bulging or perforations of the tympanic membrane), and rats without DPOAE in any of the studied frequencies (18 kHz)

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Slides were stored for 2448 h in the dark at RT to dry