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Huang, C.S., M.E

Excessive amounts of APAP metabolism in the liver generate a toxic intermediate, N-acetyl-p-benzoquinone imine (NAPQI), mainly through the activity of cytochrome P450 enzymes CYP2E1 and CYP1A2

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These data enhance the notion that even if, in theory, either peaks relative to GS-NEM conjugate can be used for calculations, the peak eluting at about 4.29 min r.t