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However, organic solvents may disrupt the secondary and tertiary structures of proteins, leading to a decrease in their biological activity

This occurs through multiple signaling cascades including the cAMP responsive element-binding protein (CREB) pathway

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Because K63-linked polyubiquitination is known to be involved in various cellular activities, such as signal transduction, protein trafficking, proteinprotein interaction and DNA repair 51,52 , it is possible that K63-linked GSK3 polyubiquitination by Pellino3 influences GSK3-mediated signal transduction in APAP-induced liver injury and does not affect the stability of the GSK3 protein