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Most patients prefer 29-31 gauge for comfort

doi: 10.2147/NDT.S212361

This steady state is observed a few seconds after agitation when the reading remains constant for 30 seconds or more

Add the reconstitution volume slowly along the vial wall rather than directing the stream onto the lyophilised cake, swirl gently until fully dissolved, and dont vortex as mechanical agitation can cause peptide and protein aggregation