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GenJet In Vitro DNA Transfection Reagent and buffer were purchased from Tebu-bio (Heerhugowaard, Netherlands)

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Expression levels of proteins such as IRP1 and TfR were increased in FRDA fibroblasts, while ferritin and mitochondrial ferritin were downregulated, suggesting a deficiency in intracellular iron availability

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At the time of this report, it was understood that brain iron accumulates naturally with age in humans, and mostly within the first 30 years of life