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After 48 h, the mice were sacrificed, and the heart, liver, spleen, lung, kidney, and tumor tissues were subsequently isolated, weighed, and nitrated for further analysis

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Previous work revealed an upregulation of activated T cells up to 8 months after infection 36

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Hossain MA, Piyatida P, da Silva JAT, Fujita M (2012) Molecular mechanism of heavy metal toxicity and tolerance in plants: central role of glutathione in detoxification of reactive oxygen species and methylglyoxal and in heavy metal chelation

Light exposure should be minimized, so its best to store the vials in their original carton or in an opaque container in the fridge