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This rapid and excessive influx of neutrophils leads to persistent inflammation and damage to the infected tissue by releasing ROS, proteinase (such as elastase and cathepsin G), myeloperoxidase, and metalloproteinases (103, 104)

the culture medium was subsequently replaced with DMEM-LG supplemented with additional 25 mM glucose (1.08337.1000, Merck Millipore, Darmstadt, Germany) and incubated for 24 h

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216 In addition, PKC also facilitated occludin and ZO-1 assembly via the phosphorylation of Thr residues at the C-terminus of occludin