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Incubation of these inhibitors with TGR in the absence of NADPH led to significantly attenuated enzyme inhibition compared to inhibition resulting from incubation in the presence of NADPH (Fig

2.15 Western blot analysis Cells and tissues were lysed in RIPA buffer (Solarbio, Beijing, China) containing a proteinase inhibitor cocktail (Beyotime, Beijing, China) and quantified using the BCA kit (Solarbio, Beijing, China)

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Cherraben, S., Scelle, J., Hasenknopf, B., Vives, G

After reconstitution, aliquot and keep at 20 C to maintain the stability of the copper complex

He X, Gao X, Xie W