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Cells were rinsed twice in PBS and then lysed in M-Per buffer (78503, ThermoFisher Scientific) containing Halt Phosphatase Inhibitor (78428, ThermoFisher Scientific) and Halt Protease Inhibitor (78437, ThermoFisher Scientific), both diluted 1:100

Diterbutyl phthalate attenuates osteoarthritis in ACLT mice via suppressing ERK/c-fos/NFATc1 pathway, and subsequently inhibiting subchondral osteoclast fusion

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(2020) noted that the application of Zn (10 mg/L) improved Chl, Tr, Gs, Pn, and water use efficiency (WUE) by 37%, 36%, 6%, 40%, 6%, and 126%, respectively, in B

stepwise or concerted mechanism

115 Furthermore, a study in a neonatal rat model found that caffeine attenuated hyperoxia-mediated reductions in the expression of transcription factor mRNA that was important to the process of neurogenesis