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In summary: expect to use it for a few months and to see noticeable improvement by the end of the treatment course (around 2-3 months)

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Studies using human aortic endothelial cells (HAECs), human vascular smooth muscle cells (HVSMCs) and mouse models have shown that TMAO treatment increases phosphorylation of p38, JNK and ERK1/2 [38, 132]

Lysates were incubated at 20 C for 30 min, followed by at least 2 h at 80 C, and cleared by centrifugation

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