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The reactivity toward DTNB (50 M) was followed spectrophotometrically at 412 nm where TNBS - absorbs ( TNBS - = 11800 M 1 cm 1 at pH 5.0) in 0.1 M sodium acetate buffer pH 5.0, 0.12 M urea (25 C)

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The flow rate of mobile phase was 0.3 mL/min, which was constituted by solvents A (H 2 O+0.1% acetic acid) and B (Acetonitrile+0.1% acetic acid)

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