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10.1371/journal.pone.0223435 40 LeeJ

Adoptive transfer of vaccine-induced peripheral blood mononuclear cells to patients with metastatic melanoma following lymphodepletion

All cells except for hNF-1 were grown and maintained as monolayer cultures at 37 C in 5% CO 2 in a humidified atmosphere, using DMEM (high glucose with l-glutamine and phenol red, Fujifilm Wako Pure chemical corporation), supplemented with penicillinstreptomycin solution (100) (Fujifilm Wako Pure Chemical Corporation), and 10 vol% FBS (Sigma-Aldrich Co., Llc.)

Ann Intern Med 104:399 Wootton FT, Lee WM (1990) Acetaminophen hepatotoxicity in the alcoholic

E.TosakulwongN.LiesingerA

Operationally, 200 l conditioned medium from a 500 l culture well was transferred to a 0.6 ml sterile tube