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The sequencing library for each sample was prepared using the same method described above for the plasmid DNA pools, with custom indices for the second-round PCR detailed in Supplementary Table 3

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Not only is it critical for endurance athletes to show endurance, the higher VO2 max is associated with longevity and cardiovascular risk

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Nasal spray protocol: Dose: 200-500 mcg per application Frequency: 1 to 2 times daily Duration: 4 to 8 weeks Nasal delivery is particularly interesting for researchers focused on neurological applications, as the nasal route provides a potential pathway to bypass the blood-brain barrier through the olfactory and trigeminal nerve pathways