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For macrophage generation 50 ng/mL M-CSF (PeproTech) was added every 3 days for at least 9 days

Redness and swelling develop as a result of increased blood flow from newly formed capillary blood vessels

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Emerging evidence suggests that microglia, as key neuroimmune modulators within the CNS, influence the incidence and progression of cardiovascular diseases, including hypertension, myocardial infarction, and ischemia/reperfusion injury, through mechanisms potentially linked to altered autonomic nervous system activity (Wang M

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At the molecular level, the circadian clock functions through a transcription-translation feedback loop (TTFL), with about 24 h periodicity [6]