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Perduca AE

Clemen R, Dethloff W, Berner J, Schulan P, Martinet A, Weltmann KD, et al

Yes, methotrexate is most conveniently injected by yourself, a friend or family member

Moreover, El-halfawy et al

Mice were first acclimated for 2 hours prior to dissection to help control for activity-induced changes and all dissections were performed at zeitgeber hours ZT6 to ZT11 to control for potential circadian affects