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Aseptic Filling: Under strict aseptic conditions, the sterile solution is filled into pre-sterilized glass vials

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Bone-marrow derived macrophage generation 812 week old C57BL/6J mice (weighing approximately 2025 g) were euthanized by gradual-fill CO 2 inhalation (2030% chamber volume per minute) without prior anesthesia, in accordance with the HPRA, Ireland, Directive 2010/63/EU of the European Parliament, the ARRIVE guidelines, and the Guide for the Care and Use of Laboratory Animals

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The emerging field of lipidomics