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Studies in rats undergoing subtotal nephrectomy (5/6Nx) have shown that IGF-1 administration failed to increase GFR in this model of renal insufficiency.66 Close to this finding, recombinant IGF-1 administration induces a dose-dependent decrease in plasma levels of insulin, C-peptide, amino acids and glucose in patients undergoing hemodialysis or peritoneal dialysis

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Cell debris was removed by centrifugation (20 min, 4 C, 15,000 g ), and the protein concentration of the supernatants was determined using the Pierce BCA (Thermo Fisher Scientific) with a bovine serum albumin standard (yielding an average of 5.8 g/L protein)

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