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The refined subtrees were midpoint-rooted using FastRoot [58], and tips were initially clustered using TreeCluster v1.0.4 (branch length threshold = 0.02, single linkage) [59]

Pathway analysis of significantly altered proteins in muscle revealed that APOE4 affected the expression of pathways involved in metabolism, including insulin secretion in females, sulfur amino acid metabolism in males, and mitochondrial dysfunction in both sexes (Supplementary Table 1)

This allows the liver to function more efficiently, supporting weight management, boosting the immune system, and improving the bodys natural detoxification processes

99 , E11831190 (2014)