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As previously outlined [4], increased levels of proinflammatory cytokines, oxidative damage, increased cyclo-oxygenase 2 (COX-2) production, increased translocation by Gram-negative enterobacteria and so on, can generate F&S symptoms, including fatigue, a flu-like malaise, pain, symptoms of irritable bowel syndrome, and neurocognitive disorders

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Immunofluorescence staining demonstrated that ZO-1 localization at the cell borders was markedly disrupted and fragmented in LPS-treated IEC-6 cells, indicating severe tight junction damage