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Bisulphite converted DNA was amplified using primers designed to the relevant target regions (see Additional file 2: Methods) and pyrosequencing performed using Qiagen PyroMark software and system

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They are rarely a sign of anything serious but can cause pain and discomfort when eating, drinking, or brushing your teeth

Gray literature was not actively sought but considered if eligibility criteria were met

Kim S, Goel R, Kumar A, Qi Y, Lobaton G, Hosaka K et al

From there, the nutrient is gradually released into the bloodstream over time