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Neutrophils were removed from co-culture with cancer cells after 3 h of incubation at 37 degrees, washed, counted and 50,000 neutrophils re-suspended in 50 l 1X PBS, and placed in a black 96 well plate (Corning 3904), with each condition performed in triplicate

From a clinical perspective, these results suggest that nebulized levofloxacin is a safe and effective therapeutic option for patients with CBI and chronic structural lung diseases, especially NCFB, particularly those with frequent exacerbations and prior treatment with other IAs

Increased lipid peroxidation, a hallmark of ferroptosis, is observed in tubules within IRI kidneys [32, 33], further aligning with the pathological involvement of ferroptosis in renal IRI

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