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For example, it has been shown that an increase in GSSG levels is required for the activation of defense signaling in response to infections by powdery mildews and bacteria ( The fact that GSH overproduction restores wild type levels of TMV resistance in SA-deficient NahG tobacco implies that GSH and SA may induce partially overlapping defense pathways and high levels of GSH may confer TMV resistance by enhancing normal SA accumulation in wild type plants

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For fluorescence microscopy studies, HL-60 cells and HepG2 cells (seeding densities 1 10 4 cells/well and 8 10 4 cells/well, respectively) were placed in serum-free medium to facilitate their adhesion to coverslips and cultured on lysine-coated 8-well chamber slides (IBIDI) following the procedure detailed by 2.8 Cell surface binding, endocytosis, and downregulation of TfRs For surface binding and endocytosis of AF-labeled proteins, the cells were rinsed twice with ice-cold HEPES-buffered, serum-free media, pH 7.4, and incubated for 1 h on ice with either 500 nM hemeAF546HPX, hemeAF546HPX, or AF488 holo-human Tf

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