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Abbreviations AP-MS: Affinity purification - mass spectrometry ATP: Adenosine triphosphate CAF: Central Analytical Facilities ChIP-seq: Chromatin immunoprecipitation followed by sequencing DAVID: Database for Annotation, Visualization and Integrated Discovery DNA: Deoxyribonucleic acid ESCRT: Endosomal sorting complex required for transport FDR: False Discovery Rate FLAG: DYKDDDDK polypeptide tag GFP: Green fluorescent protein GO: Gene Ontology IP: Immunoprecipitation LB: Luria-Bertani LFQ: Label-free quantification MRM: Multiple reaction monitoring MS/MS : Tandem mass spectrometry MYH7 : Anti-human myosin heavy chain 7 PBS: Phosphate buffered saline PCR: Polymerase Chain Reaction PE: proline-glutamic acid PEP : Posterior Error Probability Scores PPE : proline-proline-glutamic acid RNA-seq: RNA sequencing Sec: General secretion pathway SRM: Selected reaction monitoring SRP: Signal recognition particle TEAB: Triethylammonium bicarbonate USA: United States of America WT MS : Wild type Mycobacterium smegmatis ESX-3 MS : ESX-3 knock-out M