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Ju, X., Fan, D., Kong, L., Yang, Q., Zhu, Y., Zhang, S., et al., Molecules , 2021, vol

The control group received saline solution

The purified receptor proteins were dialyzed to storage buffer and they were stored at -80C until their use for fluorescence polarization peptide binding assay

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doi: 10.1016/j.actbio.2022.11.057 232 YuMLiuWLiJLuJLuHJiaWet al

Briefly, HepG2, Hepa1-6, and KYSE-150 cells subjected to various treatments were collected and incubated with 1 mL 2.5 M MitoSOX Red at ambient temperature for 20 min