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However, it is noteworthy that knockdown of NNMT does not seem to affect NAD + levels in the liver, indicating potential divergent regulatory mechanisms of NNMT in these tissues and suggesting the existence of redundant pathways in NAD + metabolism regulation (Kraus et al., 2014)

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treatment of the adipocytes with the NNMT inhibitor 5-amino-1MQ resulted in a concentration-dependent increase in the NAD+ levels with concentrations in the range of 1-60 M resulting in 1.2-1.6-fold increase in NAD+ levels relative to control adipocytes