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The specific signal for each sample was calculated after subtracting the background signal obtained for antigen-free wells

For nuclei extraction, the cells were incubated with 0.1% (v/v) digitonin (Promega, USA) for 3 min at 4 C and centrifuged for 10 min at 500 x g at 4 C with 1 mL ATAC-RSB, containing 0.1% (v/v) Tween-20

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These effects were particularly pronounced when EAAT3 was co-overexpressed with xCT [46]