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Liquid chromatography and tandem mass spectrometrycolistin quantification in kidney and serum One whole kidney was resuspended in ice-cold PBS and homogenized in a motor-driven tightly fitting glass-Teflon Potter grinder operated at 3,000 rpm and centrifuged at 4 C for 20 min, at 2,000 g av

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The mean liothyronine (T3) concentration was 52 mcg/L (range 32 to 109 mcg/L) and the mean reverse T3 concentration was 65 mcg/L (range 21 to 180 mcg/L)