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First, an N-terminal 13-amino acid extension was added

This requires computationally demanding enhanced-sampling MD techniques such as alchemical free energy calculations or funnel-shaped restrained metadynamics with Hamiltonian replica-exchange (fun-SWISH) [105], which are left out for further, more in-depth studies

ALOX15 KD did, however, lead to decreased extracellular GSSG ( P = 0.021), with marginal but insignificant increases in extracellular GSH:GSSG ( P = 0.083)

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10.1111/cbdd.13957 88