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HUVECs were maintained in 10% fetal bovine serum (FBS) (10270-106, Gibco, USA) at 37 C and 5% CO 2

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Several studies have highlighted the potential of glutamine supplementation in enhancing the anti-cancer immune response, presenting a compelling avenue for improving the efficacy of immunotherapeutic approaches in cancer treatment [73]

Even though PE patients experience elevated blood pressure, their overall blood and plasma volumes are reduced 33,34 , and in such hypovolemic conditions, body fluids are distributed more into the tissue interstitium than into the vasculature, indicating capillary leakage 35

To demonstrate this principle, we first analyzed 293T cell samples labeled with isotopic tracers such as [U- 13 C]-glutamine and [U- 13 C]-glucose using a high-resolution mass spectrometer

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