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The endothelial glycocalyx restricts cell-vessel interactions and attenuates the adhesion of leukocytes to cell adhesion molecules like ICAM-1 and VCAM-1 [43, 50, 51]

The protocol from Celsis for assessing microsomal- dependent drug metabolism was followed with minor adaptations

Harting TP, Stubbendorff M, Hammer SC, Schadzek P, Ngezahayo A, Murua Escobar H, et al

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35 , 1316 (1996) Tinjac, D.A., Martnez, F., Almanza, O.A., Jouyban, A., Acree, W.E., Jr.: Solubility of meloxicam in aqueous binary mixtures of formamide, N -methylformamide and N , N -dimethylformamide: determination, correlation, thermodynamics and preferential solvation

At the cellular level in the brain abnormalities are discernible years before the disease becomes clinically manifest, but these can only be visualized microscopically following biopsy