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Of each homogenate, 100 l was transferred to a 96-well PIXUL plate and sonicated with a PIXUL Multisample sonicator (Active Motif) for 10 minutes with default settings (Pulse 50 cycles, PRF 1 kHz, Burst Rate 20 Hz)

The AMPK-SIRT1 pathway regulates autophagy by inducing the assembly of autophagosomes through the increased expression of microtubule-associated protein 1A/1B-light chain 3, a protein associated with autophagosome membranes [68]

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Jiang L, Hu X, Jin J, et al

However, LDHA has also been shown to metabolize alpha-ketoglutarate (-KG) to the L-(S)-enantiomeric form of 2-HG (L-2-HG), with similar inhibitory effects on histone demethylation ( Figure 1 IDH-mutant gliomas tend to have a missense mutation at codon 132 of IDH1, most commonly R132H, or at codons R140 or R172 of IDH2, such as R140Q or R172K