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The ROIs were used to measure the fluorescent intensity ratio in each cell

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It added that colon cleansing procedures, or enemas, a common method of detoxing, "may have side effects, some of which can be serious." An enema is a medical procedure in which a liquid or gas is injected into the colon through the rectum, usually with the intent of stimulating a bowel movement

Subsequently, local refinement and local CTF refinement were performed with a mask generated from the reconstructed volume around the 5 f axis, producing a final density map with a resolution of 3.90