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3A) was assembled, using the one-step SLIC method 60 , from five PCR fragments generated from suitable plasmid templates with 1525 bp mutual overlaps, thus introducing the following changes compared with the preceding pSB15 vector: (i) replacement of the constitutive pro S promoter for the engineered PylRS gene by the inducible ara BAD promoter, including the ara C repressor gene, (ii) replacement of the tet p/o for the POI-encoding region, here sfGFPa39, by the lac UV5 p/o , including the lac I repressor gene, and (iii) removal of the tet R repressor cistron downstream of the bla ( amp r ) gene and replacement by the coding region for chloramphenicol acetyltransferase ( cam r ) further harboring an amber stop codon at amino acid position 112

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Kim et al ., Reference Kim, Ahn, Kim, Bae, Kwon, Kang, Yang, Sohn and Kong2016), previous studies have suggested they were likely absent from cestodes (Iriarte et al ., Reference Iriarte, Arbildi, La-Rocca, Musto and Fernndez2012

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