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Coverslips were kept in the dark at RT for one night, then they were stored at 4 C

Studies with specific inhibitors of p44/42 and JNK support that the TNF-mediated increase in lipolysis is largely attributed to a reduction in perilipin levels in adipocytes (104, 105), and lower levels of perilipin have been found in adipose tissues from obese subjects (135)

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Divyashree M, Mani MK, Reddy D, Kumavath R, Ghosh P, Azevedo V