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Therefore, despite coppers promoting effects on cell survival and proliferation, and the dependence of tumor cells on copper, inducing copper accumulation in cancer cells can specifically induce the production of higher levels of ROS in tumor tissues based on the sensitivity of cancer cells to ROS and the higher concentration of copper ions in cancer tissues

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ROS are produced by several enzyme systems, including NADPH oxidase (Nox), xanthine oxidase, endothelial nitric oxide synthase, lipoxygenases and myeloperoxidase [10]