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While the removal of your gallbladder alleviated your pain and discomfort, it did absolutely nothing to improve the production of bile and the flow of your bile

For immunohistochemistry, after dewaxing and hydration, the sections were incubated with 3% hydrogen peroxide-methanol solution at room temperature for 15 min to quench endogenous peroxidase activity and 1 mmol/l levamisole for 20 min to inhibit endogenous alkaline phosphatase activity