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The colon lumen was reached by inserting a polyethylene cannula with a 4 mm diameter via the anus and advancing it such that its tip was 68 cm proximal to the anus

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Such increased permeability can also stimulate immune cells such as the macrophages and dendritic cells to release inflammatory cytokines, and enhance antigen presentation for possible autoimmune conditions (Ullah et al., 2024)

17 DuvoixA.MorceauF.DelhalleS.SchmitzM.SchnekenburgerM.GalteauM

6.5.3 Function-linked release tests To bridge identity content function, MFH products intended for PD research should adopt mechanism-linked release assays (e.g., Nrf2/ARE reporter activation, NF-B nuclear-translocation inhibition in microglia, protection of human iPSC-derived TH + neurons under oxidative stress) in addition to pharmacopeial quality tests (see Table 4 in the main text)

The rats were bred in the animal house of the facility of Basic Medical Sciences, Osun State University, Osogbo, Osun State