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In this paper, the authors provide experimental evidence for the axon-protective benefits of remyelination and hence the likely therapeutic benefits of remyelination-enhancing treatments

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Kit Components Pre-coated 96-well microplate Wash Buffer Positive Control Biotinylated Detection Antibody Biotinylated Anti-Positive Control HRP-Streptavidin concentrate TMB One-Step Substrate Stop Solution Assay Diluent Sample Diluent Additional Materials Required Microplate reader capable of measuring absorbance at 450 nm Precision pipettes to deliver 2 L to 1 mL volumes Adjustable 1-25 mL pipettes for reagent preparation 100 mL and 1 liter graduated cylinders Absorbent paper Distilled or deionized water Assay Procedure Summary Prepare all reagents, samples and standards as instructed

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